Scientists develop new test for ‘pluripotent’ stem cellsMarch 7th, 2011 - 4:47 pm ICT by ANI
London, Mar 7 (ANI): Scripps Research Institute researchers have developed a quality control diagnostic test that is likely to make it easier for scientists to determine whether their cell lines are normal pluripotent cells.
“Many scientists are unhappy with the current gold standard for testing for pluripotency, called the teratoma assay,” said Scripps Research molecular biologist Jeanne Loring, principal investigator of the study.
“The teratoma assay requires animal testing and a time span of six to eight weeks before scientists can prove that they have a pluripotent stem cell line. In addition, this method is technically challenging and difficult to standardize,” he said.
The new test, called ‘PluriTest,’ meets the need for a cost-effective, accurate, animal-free alternative to the teratoma assay for assessing pluripotency.
Using microarray technology, which enables the simultaneous analysis of thousands of different DNA sequences, the Scripps Research team created a large database of information about all the genes that are active in hundreds of normal human embryonic and induced pluripotent stem cells and a variety of non-pluripotent cell lines.
For PluriTest, this database was used to create a detailed molecular model of a normal pluripotent stem cell line.
“Unlike diagnostic tests that use small sets of biomarkers to examine cells, the molecular model approach uses all of the thousands of pieces of information in a microarray,” said Loring.
Scientists upload raw data straight from a single microarray analysis to the PluriTest website and learn within 10 minutes whether their cell line is pluripotent.
An additional feature of the PluriTest diagnostic test is that it can show whether a cell that is pluripotent is different in some way from the normal model pluripotent cell line.
Franz-Josef Mueller, a first author of the study, said, “Scientists are making new induced pluripotent stem cell lines at a rapid pace to understand human disease, test new drugs, and develop regenerative therapies. Thousands of induced pluripotent stem cell lines have already been generated and soon there will be many more thousands. PluriTest is designed to enable the growth of this technology.”
The findings have been published in the journal Nature Methods. (ANI)
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