New method to produce native-like spider silk foundJuly 28th, 2010 - 3:26 pm ICT by ANI
Washington, July 28 (ANI): Researchers have devised a new method to produce native-like spider silk that is light-weighted while as strong and tough as steel or Kevlar.
The method devised by a team led by Sang Yup Lee at the Korea Advanced Institute of Science and Technology (KAIST) in Daejeon, is very similar to what spiders essentially do-first, expression of recombinant silk proteins; second, making the soluble silk proteins into water-insoluble fibers through spinning.
For the successful expression of high molecular weight spider silk protein, researchers pieced together the silk gene from chemically synthesized oligonucleotides, and then inserted it into the expression host (in this case, an industrially safe bacterium Escherichia coli which is normally found in our gut).
Initially, the bacterium refused to the challenging task of producing high molecular weight spider silk protein due to the unique characteristics of the protein, such as extremely large size, repetitive nature of the protein structure, and biased abundance of a particular amino acid glycine.
“To make E. coli synthesize this ultra high molecular weight (as big as 285 kilodalton) spider silk protein having highly repetitive amino acid sequence, we helped E. coli overcome the difficulties by systems metabolic engineering,” said Lee.
His team boosted the pool of glycyl-tRNA, the major building block of spider silk protein synthesis.
“We could obtain appreciable expression of the 285 kilodalton spider silk protein, which is the largest recombinant silk protein ever produced in E. coli. That was really incredible,” said a co-author of the study.
But this was only step one. The KAIST team performed high-cell-density cultures for mass production of the recombinant spider silk protein.
Then, the team developed a simple, easy to scale-up purification process for the recombinant spider silk protein.
The purified spider silk protein could be spun into beautiful silk fiber.
To study the mechanical properties of the artificial spider silk, the researchers determined tenacity, elongation, and Young’s modulus, the three critical mechanical parameters that represent a fiber’s strength, extensibility, and stiffness.
Importantly, the artificial fiber displayed the tenacity, elongation, and Young’s modulus of 508 MPa, 15pct, and 21 GPa, respectively, which are comparable to those of the native spider silk.
“We have offered an overall platform for mass production of native-like spider dragline silk. This platform would enable us to have broader industrial and biomedical applications for spider silk. Moreover, many other silk-like biomaterials such as elastin, collagen, byssus, resilin, and other repetitive proteins have similar features to spider silk protein. Thus, our platform should also be useful for their efficient bio-based production and applications,” concluded Lee. (ANI)
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Tags: amino acid glycine, amino acid sequence, bacterium escherichia coli, cell density, daejeon, e coli, insoluble fibers, kaist, kilodalton, korea advanced institute, korea advanced institute of science and technology, metabolic engineering, molecular weight, protein structure, protein synthesis, repetitive nature, silk gene, silk protein, silk proteins, spider silk