MicroRNAs could increase amputation risk in diabeticsJanuary 13th, 2011 - 2:09 pm ICT by ANI
Washington, Jan 13 (ANI): A new study has discovered that micro-RNA, one of the smallest entities in the human genome, could increase the risk of limb amputation in diabetic patients who have poor blood flow.
Andrea Caporali and colleagues at the University of Bristol have shown in an experimental cell study that conditions mimicking diabetes and a lack of blood supply to a tissue increased a particular miRNA (miRNA-503) and impaired the ability of endothelial cells, which line the interior surface of blood vessels.
Micro-RNAs (miRNAs) are small sections of ribonucleic acid (RNA) that can inhibit many genes.
Alternatively, slowing down miRNA-503 improved the capability of endothelial cells to duplicate and form into networks of small blood vessels.
The researchers showed that microRNA-503 reduces cell growth and prevents the formation of blood vessels by direct binding and inhibition of cyclin E1 and Cdc25 mRNA.
The team subsequently investigated miR-503 and target gene expression in muscular specimens from the amputated ischaemic legs of diabetic patients.
As controls, calf biopsies of non-diabetic and non-ischemic patients undergoing saphenous vein stripping were used. In diabetic muscles, miR-503 expression was remarkably higher, and plasma miR-503 levels were also elevated in the diabetic subjects.
Finally, using mouse models of diabetes and limb ischaemia, the researchers found that inhibition of the miRNA-503 (using a “decoy miRNA”) could restore-post-ischaemic blood flow recovery.
The study has been published online in Circulation: Journal of the American Heart Association. (ANI)
- Micro-RNA that regulates insulin in obesity identified - Apr 01, 2011
- Scientists convert skin cells to stem cells more effieciently - Feb 03, 2011
- Boffins identify 'gas pedal', 'brake' for uncontrolled cell growth - Aug 02, 2010
- RNA-based drugs may prevent premature labour - Nov 16, 2010
- New discovery could shrink dengue-spreading mosquito population - Dec 03, 2010
- New discovery may help in the fight against ovarian cancer - Feb 04, 2011
- New discovery shows promise against pancreatic cancer - Dec 16, 2010
- Stem cell patch may improve function after heart attack - Nov 16, 2010
- Scientists uncover clues to cancer development in AIDS patients - Jun 18, 2009
- Micro-RNA decides malignancy of lung cancer - Sep 09, 2010
- Key step in body's ability to make red blood cells found - Aug 01, 2010
- Lung cancer culprit could offer therapeutic target - Sep 14, 2010
- Indian-origin scientist finds genetic switch that may help treat vascular diseases - Jul 06, 2009
- Autism effects may be reversible - Apr 24, 2010
- New way to slow growth of deadly skin cancer - Jul 09, 2010
Tags: american heart association, andrea caporali, biopsies, blood supply, circulation journal of the american heart association, decoy, diabetic patients, diabetic subjects, endothelial cells, human genome, interior surface, ischaemic, limb amputation, limb ischaemia, mirna, mouse models, poor blood flow, rnas, target gene expression, university of bristol