Thaindian News

Washington, Dec 19 (IANS) Researchers have genetically modified Escherichia coli, a food poisoning pathogen, to create unusually long-chain alcohols, essential in the production of high-energy biofuels. Longer-chain alcohols, with five or more carbon atoms, pack more energy into a smaller space and are easier to separate from water, making them less volatile and corrosive than the commercially available biofuel ethanol.

The greater the number of carbon atoms, the higher the density of the biofuel. Ethanol, most commonly made from corn or sugarcane, contains only two carbon atoms.

“Previously, we were able to synthesise long-chain alcohols containing five carbon atoms,” said James Liao, University of California Los Angeles (UCLA) professor of chemical and biomolecular engineering.

“We stopped at five carbons at the time because that was what could be naturally achieved. Alcohols were never synthesised beyond five carbons.”

“Now, we’ve figured out a way to engineer proteins for a whole new pathway in E. coli to produce longer-chain alcohols with up to eight carbon atoms,” he added.

Organisms typically produce a large number of amino acids, which are the building blocks of proteins. In their research, Liao’s team examined the metabolism of amino acids in E. coli and changed the metabolic pathway of the bacterium by inserting two specially coded genes.

One gene, from a cheese-making bacterium, and another, from a type of yeast often used in baking and brewing, were altered to enable E. coli’s amino acid precursor, keto acid, to continue the chain-elongation process that ultimately resulted in longer-chain alcohols, said an UCLA release.

“This research is significant for two reasons,” said Liao, the study’s lead author. “From a scientific standpoint, we wanted to show that we can expand nature’s capability in making alcohol molecules. We showed we are not limited by what nature creates.”

“From an energy standpoint, we wanted to create larger, longer-chain molecules because they contain more energy. This is significant in the production of gasoline and even jet fuel,” he added.

“We used E. coli because the genetic system is well known, it grows quickly and we can engineer it very easily,” said co-author Kechun Zhang, a UCLA postdoctoral researcher.

The new protein and metabolic engineering method will be highlighted in the Dec 30 issue of Proceedings of the National Academy of Sciences.